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KMID : 1161520020060040341
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Animal Cells and Systems
2002 Volume.6 No. 4 p.341 ~ p.347
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Changes in profiles of major proteins in encysting acanthamoeba castellanii
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Park Joon-Tae
Jeong Young-Eui
Ahn Tae-In
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Abstract
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The life cycle of Acanthamoeba is comprised of two distinct stages, trophozoite and cyst. During periods of stress, trophozoites undergo cellular differentiation into cyst. In order to understand the cellular differentiation, we followed changes in profiles of major proteins by 2D?PAGE and ubiqui?tinated proteins by immunoblotting with anti?ubiquitin (Ub) monoclonal antibody (mAb) as a probe. We observed 51 proteins present in trophozoite were lost with the encystment. We found that 43 proteins within 24 h, and 8 proteins in 96 h of encystment. Among them, 17 proteins were stained with anti?Ub mAb. In cysts, 16 proteins including 2 anti?Ub mAb?reactive proteins were newly synthesized. Four proteins were newly detected in 24 h?cyst and disappeared in 96 h?cyst, one protein was synthesized in 24?96 h?cyst and disappeared in 168 h?cyst, and 11 proteins appeared upon encystment and were present in all cyst stages. We identified a cyst specific 33 kDa protein as subtilis in?like serine proteinase by N?terminal sequencing. Identification of these proteins lost and newly synthesized with encystment would improve our understanding of cysting protozoan parasites.
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KEYWORD
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Cellular differentiation, Ubiquitin, Encystment, Acanthamoeba, Protein profile
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